Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) antibody (Red).)
Rabbit DARPP32 Polyclonal Antibody | anti-DARPP32 antibody
DARPP32 Antibody (Thr75)
Gene Names
PPP1R1B; DARPP32; DARPP-32
Applications
Immunofluorescence
Purity
Affinity Chromatography
Synonyms
DARPP32; Polyclonal Antibody; DARPP32 Antibody (Thr75); Rabbit Polyclonal DARPP32 Antibody (Thr75); Polyclonal DARPP32 antibody; Anti-DARPP32 antibody; DARPP-32 antibody; DARPP32 antibody; dopamine and cAMP regulated phosphoprotein antibody; dopamine and cAMP-regulated neuronal phosphoprotein 32 antibody; Dopamine- and cAMP-regulated neuronal phosphoprotein antibody; FLJ20940 antibody; PPP1R1B antibody; PPR1B antibody; Protein phosphatase 1 regulatory subunit 1B antibody; protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody; protein phosphatase 1 regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein DARPP-32) antibody; anti-DARPP32 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
DARPP32 antibody (Thr75) detects endogenous levels of DARPP32 only whenphosphorylated at threonine 75
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
204
Applicable Applications for anti-DARPP32 antibody
Immunofluorescence (IF)
Application Notes
IF: 1:100-1:200
Immunogen
DARPP32 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human DARPP32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) antibody (Red).)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) antibody (Red).)
Related Product Information for anti-DARPP32 antibody
DARPP32 is a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions.
Product Categories/Family for anti-DARPP32 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
dopamine and cAMP-regulated neuronal phosphoprotein
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 1B
NCBI Official Symbol
PPP1R1B
NCBI Official Synonym Symbols
DARPP32; DARPP-32
NCBI Protein Information
protein phosphatase 1 regulatory subunit 1B
NCBI Description
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on DARPP32
Similar Products
Product Notes
The DARPP32 (Catalog #AAA5313619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DARPP32 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). IF: 1:100-1:200. Researchers should empirically determine the suitability of the DARPP32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARPP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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