Mouse anti-Human, Mouse KAP-1 Monoclonal Antibody | anti-kap-1 antibody

KAP-1 (KRAB-Associated Protein 1, TF1B, TIF1B, TIF1-beta, Transcriptional Intermediary Factor 1-beta, TRIM28, Tripartite Motif-Containing 28; Nuclear Corepressor KAP-1)

Cat. Num. AAA604251

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: KAP-1; Monoclonal Antibody; KAP-1 (KRAB-Associated Protein 1; TF1B; TIF1B; TIF1-beta; Transcriptional Intermediary Factor 1-beta; TRIM28; Tripartite Motif-Containing 28; Nuclear Corepressor KAP-1); Anti -KAP-1 (KRAB-Associated Protein 1; anti-kap-1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C202
• Specificity: Recognizes human KAP-1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-kap-1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: Western Blot: 0.2-2ug/ml; Immunocytochemistry: 2-100ug/ml.; Optimal dilutions to be determined by researcher.
• Immunogen: Partial sequence of recombinant full-length protein to human KAP-1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of TRIM28 by Western blot.|Samples: Whole cell lysate from human HEK293 (25ug) and mouse NIH3T3 (25ug) cells.)

Immunostaining (IS)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS604251.)

Testing Data

Related Product Information for anti-kap-1 antibody

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Product Categories/Family for anti-kap-1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 71984142
• NCBI GeneID: 176041
• NCBI Accession #: NP_001021247.1
• NCBI GenBank Nucleotide #: NM_001026076.2
• UniProt Accession #: Q8MYQ8
• Molecular Weight: 78,788 Da
• NCBI Official Full Name: Kinesin-Associated Protein family member (kap-1)
• NCBI Official Symbol: kap-1
• NCBI Protein Information: Kinesin-Associated Protein family member (kap-1)
• UniProt Protein Name: Kinesin-associated protein protein 1, isoform a, confirmed by transcript evidence
• UniProt Gene Name: kap-1
• UniProt Entry Name: Q8MYQ8_CAEEL