Typical Testing Data/Standard Curve (for reference only)
Human Alpha 2B adrenergic receptor (ADRA2B) ELISA Kit | ADRA2B elisa kit
Human Alpha 2B adrenergic receptor (ADRA2B) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADRA2B ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADRA2B antibody and an ADRA2B-HRP conjugate. The assay sample and buffer are incubated together with ADRA2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA2B concentration since ADRA2B from samples and ADRA2B-HRP conjugate compete for the anti-ADRA2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA2B from the sample, fewer sites are left to bind ADRA2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA2B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a seven-pass transmembrane protein. This protein is a member of a subfamily of G protein-coupled receptors that regulate neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. [provided by RefSeq, Apr 2014]
Uniprot Description
ADRA2B: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.
Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 2q11.1
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; alpha2-adrenergic receptor activity; epinephrine binding
Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; cell-cell signaling; activation of protein kinase B; regulation of vascular smooth muscle contraction; negative regulation of epinephrine secretion; MAPKKK cascade; female pregnancy; angiogenesis; positive regulation of neuron differentiation; blood coagulation; positive regulation of blood pressure
Disease: Epilepsy, Familial Adult Myoclonic, 2
Research Articles on ADRA2B
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Product Notes
The Human ADRA2B adra2b (Catalog #AAA7246520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7246520 ELISA Kit recognizes Human ADRA2B. It is sometimes possible for the material contained within the vial of "Alpha 2B adrenergic receptor (ADRA2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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