Mouse Kaiso Monoclonal Antibody | anti-ZBTB33 antibody
Kaiso (Zinc Finger and BTB Domain Containing 33, ZBTB33)
Gene Names
ZBTB33; ZBTB33
Reactivity
Canine, Chicken, Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A chromatography
Synonyms
Kaiso; Monoclonal Antibody; Kaiso (Zinc Finger and BTB Domain Containing 33; ZBTB33); Anti -Kaiso (Zinc Finger and BTB Domain Containing 33; anti-ZBTB33 antibody
Host
Mouse
Reactivity
Canine, Chicken, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6A100
Specificity
Reacts with the ~95kD Kaiso protein. Reactivity is confirmed with mouse mammary adenocarcinoma cell line CSML0. Reactivity has been confirmed in MDCK (dog), NBTII (rat), HCT116 (human) and chicken embryo fibroblasts.
Purity/Purification
Affinity Purified
Purified by Protein A chromatography
Purified by Protein A chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
Applicable Applications for anti-ZBTB33 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot and ELISA.
Dilution: Immunoprecipitation: 10ug/IP reaction
Immunofluorescence: 10ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Note: Immunofluorescent assays were performed on cell lines fixed with 100% methanol at -20 degree C for 7 minutes.
Dilution: Immunoprecipitation: 10ug/IP reaction
Immunofluorescence: 10ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Note: Immunofluorescent assays were performed on cell lines fixed with 100% methanol at -20 degree C for 7 minutes.
Immunogen
Fusion protein encompassing the N-terminus of mouse Kaiso.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ZBTB33 antibody
Kaiso is a transcriptional represor that belongs to POZ-zinc finger family of transcription factors implicated in development and cancer. It binds to specific methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and it also binds to non-methylated consensus sequence 5'-CTGCNA-3'. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of represive chromatin structures in taget gene promoters. Kaiso may contribute to the repression of target genes of the Wnt signaling pathway.
Kaiso is a novel member of the BTB/POZ (Broad complex, Tramtrak, Bric à brac/Pox virus and zinc finger) zinc finger superfamily of transcription factors. Yeast 2-hybrid studies have shown that Kaiso interacts with catenin p120ctn. This interaction involves the Arm repeats of 1-7 of the p120ctn Armadillo domain and the carboxyl-terminal third of Kaiso.
Kaiso is a novel member of the BTB/POZ (Broad complex, Tramtrak, Bric à brac/Pox virus and zinc finger) zinc finger superfamily of transcription factors. Yeast 2-hybrid studies have shown that Kaiso interacts with catenin p120ctn. This interaction involves the Arm repeats of 1-7 of the p120ctn Armadillo domain and the carboxyl-terminal third of Kaiso.
Product Categories/Family for anti-ZBTB33 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transcriptional regulator Kaiso
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 33
NCBI Official Symbol
ZBTB33
NCBI Official Synonym Symbols
ZBTB33
NCBI Protein Information
transcriptional regulator Kaiso
Protein Family
Similar Products
Product Notes
The ZBTB33 (Catalog #AAA603174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Kaiso (Zinc Finger and BTB Domain Containing 33, ZBTB33) reacts with Canine, Chicken, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kaiso can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot and ELISA. Dilution: Immunoprecipitation: 10ug/IP reaction Immunofluorescence: 10ug/ml Western Blot: 1-3ug/ml ELISA: 0.1-1ug/ml Note: Immunofluorescent assays were performed on cell lines fixed with 100% methanol at -20 degree C for 7 minutes. Researchers should empirically determine the suitability of the ZBTB33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kaiso, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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