Western Blot
(Western Blot analysis using GFI1 antibodyWestern Blot showing GFI1 antibody used against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.)
Mouse GFI1 Monoclonal Antibody | anti-GFI1 antibody
GFI1 antibody
WB: 1:500-1:2000
Western Blot
(Western Blot analysis using GFI1 antibodyWestern Blot showing GFI1 antibody used against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.)
Western Blot
(Western Blot analysis using GFI1 antibodyWestern Blot showing GFI1 antibody used against HEK293 (1) and GFI1 (AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GFI1: Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2- GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin- mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1- dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells. Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2). SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA). NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
Protein type: Cell cycle regulation; Transcription factor; C2H2-type zinc finger protein; Cell development/differentiation
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: nuclear body; nuclear matrix; transcriptional repressor complex; nucleus
Molecular Function: protein binding; zinc ion binding
Biological Process: negative regulation of cell fate specification; G1/S-specific transcription in mitotic cell cycle; inner ear morphogenesis; regulation of toll-like receptor signaling pathway; cell fate commitment; transcription, DNA-dependent; viral reproduction; positive regulation of cell fate specification; mechanosensory behavior; inhibition of NF-kappaB transcription factor; auditory receptor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Disease: Neutropenia, Nonimmune Chronic Idiopathic, Of Adults; Neutropenia, Severe Congenital, 2, Autosomal Dominant
Research Articles on GFI1
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Product Notes
The GFI1 gfi1 (Catalog #AAA831629) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GFI1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:10,000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GFI1 gfi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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