Western Blot (WB)
(Western blot analysis of total (left) and T183/Y185 phosphorylated (right) JNK. HEK293 cells were either untreated, treated with phosphatase or exposed to UV radiation. T183/Y185 phosphorylated JNK levels were analysed using Mouse anti Human JNK (p Thr183/p Tyr 185) .)
Mouse anti-Human JNK Monoclonal Antibody
MOUSE ANTI JNK (pThr183/pTyr185)
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
JNK; Monoclonal Antibody; MOUSE ANTI JNK (pThr183/pTyr185); Jnk (pThr183/pTyr185) antibody|D04-7G6; anti-JNK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
D04-7G6
Specificity
JNK (pThr183/pTyr185)
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from ascites
Purified IgG prepared by affinity chromatography on Protein G from ascites
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-JNK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: Max Dilution: 1/1000
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunogen
Synthetic peptide corresponding to residues surrounding threonine 183 and tyrosine 185 of human JNK
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Fusion Partners
Spleen cells from immunised BLAB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of total (left) and T183/Y185 phosphorylated (right) JNK. HEK293 cells were either untreated, treated with phosphatase or exposed to UV radiation. T183/Y185 phosphorylated JNK levels were analysed using Mouse anti Human JNK (p Thr183/p Tyr 185) .)
Western Blot (WB)
(Western blot analysis of total (left) and T183/Y185 phosphorylated (right) JNK. HEK293 cells were either untreated, treated with phosphatase or exposed to UV radiation. T183/Y185 phosphorylated JNK levels were analysed using Mouse anti Human JNK (p Thr183/p Tyr 185) .)
Related Product Information for anti-JNK antibody
Mouse anti JNK (pThr183/pTyr185) antibody, clone D04-7G6 recognizes the c-Jun N-terminal kinase (JNK), when phosphorylated at threonine 183 and tyrosine 185. Phosphorylation of these residues is associated with the activation of JNK following exposure to enviromental stress (e.g. UV).
JNKs are members of the mitogen activated protein kinase (MAPK) family which constitute several signalling pathways. In this signalling cascade extracellular stimuli activate small GTAPases (of the Rho family) which in turn acitvate MAP2K4/MKK4 and MAP2K7/MKK7. These kinases then in turn activate JNK. JNK then interacts with a variety of downstream targets including other signalling molcules and transcription factors (Sehgal & Ram, 2013).
JNKs are members of the mitogen activated protein kinase (MAPK) family which constitute several signalling pathways. In this signalling cascade extracellular stimuli activate small GTAPases (of the Rho family) which in turn acitvate MAP2K4/MKK4 and MAP2K7/MKK7. These kinases then in turn activate JNK. JNK then interacts with a variety of downstream targets including other signalling molcules and transcription factors (Sehgal & Ram, 2013).
References
1. Sehgal, V. & Ram, P.T. (2013) Network Motifs in JNK SignalingGenes Cancer 4: 409-13
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Product Notes
The JNK (Catalog #AAA226027) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI JNK (pThr183/pTyr185) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JNK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: Max Dilution: 1/1000 Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the JNK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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