Western Blot (WB)
(Western Blot analysis of osteoprotegerin expression in various cell lines using Mouse anti Human osteoprotegerin)
Mouse anti-Human OSTEOPROTEGERIN Monoclonal Antibody
MOUSE ANTI HUMAN OSTEOPROTEGERIN
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
OSTEOPROTEGERIN; Monoclonal Antibody; MOUSE ANTI HUMAN OSTEOPROTEGERIN; Osteoprotegerin antibody|E01-10F11; anti-OSTEOPROTEGERIN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E01-10F11
Specificity
OSTEOPROTEGERIN
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from ascites
Purified IgG prepared by affinity chromatography on Protein G from ascites
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-OSTEOPROTEGERIN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: Max Dilution: 1/1000
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunogen
E Coli derived recombinant protein corresponding to aa 22-201 of human osteoprotegerin
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western Blot analysis of osteoprotegerin expression in various cell lines using Mouse anti Human osteoprotegerin)
Western Blot (WB)
(Western Blot analysis of osteoprotegerin expression in various cell lines using Mouse anti Human osteoprotegerin)
Related Product Information for anti-OSTEOPROTEGERIN antibody
Mouse anti Human osteoprotegerin, clone E01-10F11, recognizes osteoprotegerin (OPG), a cytokine receptor and member of the tumour necrosis factor (TNF) receptor superfamily. The role of OPG involves it acting as a decoy receptor for the ligand of CD254 and thereby preventing its function in osteoclastogenesis. In addition to this it may act as a decoy receptor for TNF related apoptosis inducing ligand (TRAIL) (Emery et al. 1998 and Schoppet et al. 2002). The combination of these two roles allows OPG to inhibit NFkB's regulatory effects on inflammation, skeletal, and vascular systems.
OPG is highly expressed in a wide range of tissues, including adult heart, lung, liver, prostate, ovary and bone marrow. More recently a role for OPG in cardiovascular disease has emerged and it is now clinically relevant biomarker of vascular health (Bierre, 2013).
OPG is highly expressed in a wide range of tissues, including adult heart, lung, liver, prostate, ovary and bone marrow. More recently a role for OPG in cardiovascular disease has emerged and it is now clinically relevant biomarker of vascular health (Bierre, 2013).
References
1. Emery, J.G. et al. (1998) Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL.J Biol Chem. 273 (23): 14363-7.
2. Schoppet, M. et al. (2004) Localization of osteoprotegerin, tumor necrosis factor-related apoptosis-inducing ligand, and receptor activator of nuclear factor-kappaB ligand in Mönckeberg's sclerosis and atherosclerosis.J Clin Endocrinol Metab. 89 (8): 4104-12.
3. Bjerre, M. (2013) Osteoprotegerin (OPG) as a biomarker for diabetic cardiovascular complications.Springerplus. 2: 658.
2. Schoppet, M. et al. (2004) Localization of osteoprotegerin, tumor necrosis factor-related apoptosis-inducing ligand, and receptor activator of nuclear factor-kappaB ligand in Mönckeberg's sclerosis and atherosclerosis.J Clin Endocrinol Metab. 89 (8): 4104-12.
3. Bjerre, M. (2013) Osteoprotegerin (OPG) as a biomarker for diabetic cardiovascular complications.Springerplus. 2: 658.
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Product Notes
The OSTEOPROTEGERIN (Catalog #AAA226018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN OSTEOPROTEGERIN reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OSTEOPROTEGERIN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: Max Dilution: 1/1000 Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the OSTEOPROTEGERIN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSTEOPROTEGERIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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