Mouse JAK1 Monoclonal Antibody | anti-jak1 antibody

JAK1 (Janus kinase 1, JAK-1, JAK1A, JAK1B, JTK3, Tyrosine-protein Kinase JAK1)

Cat. Num. AAA606561

• Gene Names: jak1; jak1
• Reactivity: Canine, Chicken, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: JAK1; Monoclonal Antibody; JAK1 (Janus kinase 1; JAK-1; JAK1A; JAK1B; JTK3; Tyrosine-protein Kinase JAK1); Anti -JAK1 (Janus kinase 1; anti-jak1 antibody

• Host: Mouse
• Reactivity: Canine, Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9H252
• Specificity: Recognizes human JAK1. Species Crossreactivity: mouse, rat, canine, xenopus, chicken.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 50% glycerol, BSA, 0.09% sodium azide.

• Applicable Applications for anti-jak1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:500-1:1000
• Immunogen: Recombinant protein of aa 551-776 of human JAK1. Cellular Localization: Intracytoplasmic membrane; Peripheral membrane protein.
• Positive Control: Huvec cell lysate, whole Jurkat cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunocytochemistry (ICC)

(Immunocytochemistry: A previous lot of anti-JAK1 stained human endothelial cells.)

Western Blot (WB)

(Western Blot Analysis:|Huvec lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-JAK1 (1:500 dilution of this lot). |Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates protein JAK1 (~130kD).)

Related Product Information for anti-jak1 antibody

JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common gamma-chain.

Product Categories/Family for anti-jak1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 226237498
• NCBI GeneID: 30280
• UniProt Accession #: O12990; O73880; Q7ZU16
• Molecular Weight: 132,481 Da
• NCBI Official Full Name: jak1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: jak1
• NCBI Official Synonym Symbols: jak1
• NCBI Protein Information: tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: jak1
• UniProt Entry Name: JAK1_DANRE

Uniprot Description

Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location: Endomembrane system; Peripheral membrane protein

By similarity. Note: Wholly intracellular, possibly membrane associated

Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.