Mouse anti-Human, Mouse JAK1 Monoclonal Antibody | anti-jak1 antibody

JAK1 (Janus Kinase 1)

Cat. Num. AAA608588

• Gene Names: jak1; jak1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: JAK1; Monoclonal Antibody; JAK1 (Janus Kinase 1); Anti -JAK1 (Janus Kinase 1); anti-jak1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10F612
• Specificity: Recognizes endogenous human Jak1 at ~100-120kD.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, ph 7.4, 5% trehalose. Reconstitute with 200ul of sterile PBS.

• Applicable Applications for anti-jak1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml detects JAK1 on NIH-3T3 mouse embryonic fibroblast cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line and LNCaP human prostate cancer cell line.
• Immunogen: Recombinant protein corresponding to aa32-286 of human Jak1 expressed in E. coli (P23458).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: 30ug of whole cell extracts from exponentially growing mouse NIH3T3 and human Daudi, HeLa and LNCaP (which do not express Jak1) cells were resolved by SDS-PAGE, transferred to a PVDF membrane and immunoblotted with MBS608588 (1ug/ml).)

Related Product Information for anti-jak1 antibody

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to IFN-alpha.

Product Categories/Family for anti-jak1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 226237498
• NCBI GeneID: 30280
• UniProt Accession #: O12990; O73880; Q7ZU16
• Molecular Weight: 132,481 Da
• NCBI Official Full Name: jak1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: jak1
• NCBI Official Synonym Symbols: jak1
• NCBI Protein Information: tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: jak1
• UniProt Entry Name: JAK1_DANRE

Uniprot Description

Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location: Endomembrane system; Peripheral membrane protein

By similarity. Note: Wholly intracellular, possibly membrane associated

Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.