Mouse anti-Human ISG20 Monoclonal Antibody | anti-ISG20 antibody

ISG20 (Interferon-stimulated Gene 20kD Protein, Estrogen-regulated Transcript 45 Protein, Promyelocytic Leukemia Nuclear Body-associated Protein ISG20) (Biotin)

Cat. Num. AAA6142513

• Gene Names: ISG20; CD25; HEM45
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ISG20; Monoclonal Antibody; ISG20 (Interferon-stimulated Gene 20kD Protein; Estrogen-regulated Transcript 45 Protein; Promyelocytic Leukemia Nuclear Body-associated Protein ISG20) (Biotin); anti-ISG20 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1B2-3C9
• Specificity: Recognizes human ISG20.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ISG20 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-181 from human ISG20 (AAH07922.1) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATMELYQISQRIRARRGLPRLAVSD
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (45.65kD).)

Western Blot (WB)

(Western Blot analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody. Lane 1: ISG20 transfected lysate (20.4kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ISG20 on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-ISG20 antibody

Antibodies; Interferon

References

1. Antiviral activities of ISG20 in positive-strand RNA virus infections. Zhou Z, Wang N, Woodson SE, Dong Q, Wang J, Liang Y, Rijnbrand R, Wei L, Nichols JE, Guo JT, Holbrook MR, Lemon SM, Li K.Virology. 2010 Oct 29. 2. Intracellular activation of interferon regulatory factor-1 by nanobodies to the multi-functional (Mf1) domain. Moeller A, Pion E, Narayan V, Ball KL.J Biol Chem. 2010 Sep 3.

NCBI and Uniprot Product Information

• NCBI GI #: 33871974
• NCBI GeneID: 3669
• Molecular Weight: 9,199 Da
• NCBI Official Full Name: Homo sapiens interferon stimulated exonuclease gene 20kDa, mRNA
• NCBI Official Synonym Full Names: interferon stimulated exonuclease gene 20
• NCBI Official Symbol: ISG20
• NCBI Official Synonym Symbols: CD25; HEM45
• NCBI Protein Information: interferon-stimulated gene 20 kDa protein
• Protein Family: Interferon-stimulated gene 20 kDa protein