Porcine Decorin ELISA Kit | DCN elisa kit

Porcine Decorin ELISA Kit

Cat. Num. AAA740740

• Reactivity: Porcine
• Synonyms: Decorin; Porcine Decorin ELISA Kit; DCN elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This DCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine DCN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DCN elisa kit

Principle of the Assay: DCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DCN antibody and an DCN-HRP conjugate. The assay sample and buffer are incubated together with DCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCN concentration since DCN from samples and DCN-HRP conjugate compete for the anti-DCN antibody binding site. Since the number of sites is limited, as more sites are occupied by DCN from the sample, fewer sites are left to bind DCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 609452
• Molecular Weight: 8,266 Da
• NCBI Official Full Name: decorin, partial
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; wound healing; pathogenesis; response to lipopolysaccharide; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; chondroitin sulfate catabolic process; carbohydrate metabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; aging; placenta development

Disease: Corneal Dystrophy, Congenital Stromal