Rabbit anti-Human ISG15 Monoclonal Antibody | anti-ISG15 antibody
Rabbit anti Human ISG15 Monoclonal Antibody
Gene Names
ISG15; UCRP; ISG17; ISG17/UCRP
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Synonyms
ISG15 ; Monoclonal Antibody; Rabbit anti Human ISG15 Monoclonal Antibody; anti-ISG15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
154
Applicable Applications for anti-ISG15 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 0.1-0.2 mug/mL
ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human ISG15. The detection limit for Human ISG15 is approximately 0.00245 ng/well.
ICC/IF: 10-25 mug/mL
ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human ISG15. The detection limit for Human ISG15 is approximately 0.00245 ng/well.
ICC/IF: 10-25 mug/mL
Immunogen
Recombinant Human ISG15 protein
Buffer
0.2 mum filtered solution in PBS with 5% trehalose
Preparation and Storage
This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-ISG15 antibody
Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK/lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase/signal transducer and activator of transcription (Jak/STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ISG15 ubiquitin-like modifier
NCBI Official Symbol
ISG15
NCBI Official Synonym Symbols
UCRP; ISG17; ISG17/UCRP
NCBI Protein Information
ISG15 ubiquitin-like modifier
Similar Products
Product Notes
The ISG15 (Catalog #AAA2544390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human ISG15 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISG15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 0.1-0.2 mug/mL ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human ISG15. The detection limit for Human ISG15 is approximately 0.00245 ng/well. ICC/IF: 10-25 mug/mL. Researchers should empirically determine the suitability of the ISG15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISG15 , Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
