ISG15 / G1P2 recombinant protein

Recombinant Human ISG15 / G1P2 Protein

Cat. Num. AAA2545457

• Gene Names: ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ISG15 / G1P2; Recombinant Human ISG15 / G1P2 Protein; G1P2; IFI15; IP17; UCRP; hUCRP; ISG15 / G1P2 recombinant protein

• Host: E Coli
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, pH 8.0
• Sequence Length: 165

• Application Notes: The recombinant human ISG15 consisting of 165 amino acids and has a calculated molecular mass of 17.9 kDa. It migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ISG15 / G1P2 recombinant protein

Background: Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.

Description: A DNA sequence encoding the Pro form of human ISG15 (AAH09507.1) (Met 1-Ser 165) was expressed and purified

NCBI and Uniprot Product Information

• NCBI GI #: 14550514
• NCBI GeneID: 9636
• Molecular Weight: 17,888 Da
• NCBI Official Full Name: ISG15 ubiquitin-like modifier
• NCBI Official Synonym Full Names: ISG15 ubiquitin-like modifier
• NCBI Official Symbol: ISG15
• NCBI Official Synonym Symbols: G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; UCRP; IP17; hUCRP

NCBI Description

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Uniprot Description

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; extracellular region; nucleoplasm

Molecular Function: protein binding

Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of bone mineralization; regulation of interferon-gamma production

Disease: Immunodeficiency 38