Mouse anti-Human IRF3 Monoclonal Antibody | anti-IRF3 antibody

IRF3 Mouse Monoclonal Antibody [Clone PCRP-IRF3-1E6]

Cat. Num. AAA4382021

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: IRF3; Monoclonal Antibody; IRF3 Mouse Monoclonal Antibody [Clone PCRP-IRF3-1E6]; IIAE7; MGC94729; Interferon regulatory factor 3 (IRF3); anti-IRF3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a
• Clone Number: PCRP-IRF3-1E6
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-IRF3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml
• Immunogen: Recombinant full-length human IRF3 protein
• Cellular Localization: Cytoplasm, Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm
• Positive Control: HeLa, U87 or Ramos cells.
• Hu-chromosome Location: 19q13.33
• Unigene: 289052
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunofluorescence (IF)

(Immunofluorescence Analysis of PFA-fixed HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti-mouse IgG-CF488 (green). Counterstain is phalloidin. )

Related Product Information for anti-IRF3 antibody

Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-alphaand beta) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-alphaand interferon-betagenes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3gammap48 and IFN consensus sequence-binding protein (ICSBP).

NCBI and Uniprot Product Information

• NCBI GI #: 308199450
• NCBI GeneID: 3661
• NCBI Accession #: NP_001184053.1
• NCBI GenBank Nucleotide #: NM_001197124.1
• UniProt Accession #: Q14653; Q5FBY1; A8K7L2
• Molecular Weight: 47,219 Da
• NCBI Official Full Name: interferon regulatory factor 3 isoform 4
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: IRF3
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF3
• UniProt Synonym Gene Names: IRF-3
• UniProt Entry Name: IRF3_HUMAN

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: negative regulation of interferon-beta biosynthetic process; transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; toll-like receptor signaling pathway; innate immune response; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7