Mouse anti-Human Interleukin 15 Monoclonal Antibody | anti-IL-15 antibody
Interleukin 15 (IL-15) (MaxLight 490)
Gene Names
IL15; IL-15
Reactivity
Human
Applications
Western Blot
Purity
Purified by ammonium sulfate precipitation followed by ion exchange chromatography.
Synonyms
Interleukin 15; Monoclonal Antibody; Interleukin 15 (IL-15) (MaxLight 490); anti-IL-15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4i162
Specificity
Recognizes human IL-15.
Purity/Purification
Purified by ammonium sulfate precipitation followed by ion exchange chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-IL-15 antibody
FLISA, Western Blot (WB)
Application Notes
Sandwich FLISA: 4-8ug/ml will detect 250pg/ml recombinant human IL-15 when used with Interleukin 15 (IL-15) (Biotin) (I8437-03) as the detection antibody at a concentration of 2-4ug/ml.
WB: 0.5-1ug/ml. The detection limit of recombinant human IL-15 is 2-4ng/lane under reducing or non-reducing conditions
Applications are based on unconjugated antibody.
WB: 0.5-1ug/ml. The detection limit of recombinant human IL-15 is 2-4ng/lane under reducing or non-reducing conditions
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human IL-15.
Conjugate
MaxLight490
Hybridoma
C57BL/6 F1 cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-IL-15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.9kDa (123aa), confirmed by MALDI-TOF.
NCBI Official Synonym Full Names
interleukin 15
NCBI Official Symbol
IL15
NCBI Official Synonym Symbols
IL-15
NCBI Protein Information
interleukin-15
NCBI Description
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
Research Articles on IL-15
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Product Notes
The IL-15 (Catalog #AAA6198859) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Interleukin 15 (IL-15) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 15 can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). Sandwich FLISA: 4-8ug/ml will detect 250pg/ml recombinant human IL-15 when used with Interleukin 15 (IL-15) (Biotin) (I8437-03) as the detection antibody at a concentration of 2-4ug/ml. WB: 0.5-1ug/ml. The detection limit of recombinant human IL-15 is 2-4ng/lane under reducing or non-reducing conditions Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IL-15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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