Human Cyclic AMP Response Element Binding Protein 3 (CREB3) ELISA Kit | CREB3 elisa kit

Human Cyclic AMP Response Element Binding Protein 3 (CREB3) ELISA Kit

Cat. Num. AAA2122227

• Gene Names: CREB3; LZIP; LUMAN; sLZIP
• Reactivity: Human
• Synonyms: Cyclic AMP Response Element Binding Protein 3 (CREB3); Human Cyclic AMP Response Element Binding Protein 3 (CREB3) ELISA Kit; LUMAN; LZIP; cAMP-responsive element-binding protein 3; Leucine zipper protein; Luman; Transcription factor LZIP-alpha; Transcriptionally active form; CREB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclic AMP Response Element Binding Protein 3 (CREB3).; No significant cross-reactivity or interference between Cyclic AMP Response Element Binding Protein 3 (CREB3) and analogues was observed.
• Sequence Length: 371

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Dectection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.127ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclic AMP Response Element Binding Protein 3 (CREB3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclic AMP Response Element Binding Protein 3 (CREB3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CREB3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclic AMP Response Element Binding Protein 3 (CREB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclic AMP Response Element Binding Protein 3 (CREB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclic AMP Response Element Binding Protein 3 (CREB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclic AMP Response Element Binding Protein 3 (CREB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CREB3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 22219462
• NCBI GeneID: 10488
• NCBI Accession #: NP_006359.3
• NCBI GenBank Nucleotide #: NP_006359.3
• UniProt Accession #: O43889
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 3
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3
• NCBI Official Symbol: CREB3
• NCBI Official Synonym Symbols: LZIP; LUMAN; sLZIP
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3
• Protein Family: Cyclic AMP-responsive element-binding protein
• UniProt Gene Name: CREB3
• UniProt Synonym Gene Names: LZIP; CREB-3; cAMP-responsive element-binding protein 3; N-terminal Luman; Transcriptionally active form
• UniProt Entry Name: CREB3_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-response element and regulates cell proliferation. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. This protein also plays a role in leukocyte migration, tumor suppression, and endoplasmic reticulum stress-associated protein degradation. Additional transcript variants have been identified, but their biological validity has not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

CREB3: Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response (UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. Belongs to the bZIP family. ATF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: Golgi membrane; Golgi apparatus; nuclear body; endoplasmic reticulum membrane; membrane; cell soma; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; CCR1 chemokine receptor binding; protein binding; protein homodimerization activity; DNA binding; cAMP response element binding protein binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cytoplasmic sequestering of transcription factor; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription of target genes involved in unfolded protein response; establishment of viral latency; chemotaxis; induction of positive chemotaxis; negative regulation of cell cycle; regulation of cell proliferation; reactivation of latent virus; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; positive regulation of cell migration