Western Blot (WB)
(Western Blot: Sample: Lane1: Rabbit Liver lysate; Lane2: Rat Liver lysate; Lane3: 293T cell lysate; Lane4: THP1 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Rabbit IL10 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Rabbit Interleukin 10 (IL10) Monoclonal Antibody | anti-IL10 antibody
Monoclonal Antibody to Interleukin 10 (IL10)
Gene Names
IL10; CSIF; IL-10
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Interleukin 10 (IL10); Monoclonal Antibody; Monoclonal Antibody to Interleukin 10 (IL10); Cytokine; Infection immunity; anti-IL10 antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
D4
Specificity
The antibody is a mouse monoclonal antibody raised against IL10. It has been selected for its ability to recognize IL10 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Ser19~Ser178
Sequence Length
178
Applicable Applications for anti-IL10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:500-2000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Rabbit
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rabbit Liver lysate; Lane2: Rat Liver lysate; Lane3: 293T cell lysate; Lane4: THP1 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Rabbit IL10 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rabbit Liver lysate; Lane2: Rat Liver lysate; Lane3: 293T cell lysate; Lane4: THP1 cell lysatePrimary Ab: 2ug/ml Mouse Anti-Rabbit IL10 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Interleukin-10
NCBI Official Symbol
IL10
NCBI Official Synonym Symbols
CSIF; IL-10
NCBI Protein Information
interleukin-10
UniProt Protein Name
Interleukin-10
Protein Family
UniProt Gene Name
IL10
UniProt Synonym Gene Names
IL-10; CSIF
Uniprot Description
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Research Articles on IL10
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Product Notes
The IL10 il10 (Catalog #AAA2111077) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interleukin 10 (IL10) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 10 (IL10) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:500-2000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the IL10 il10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ser19~Ser1 78. It is sometimes possible for the material contained within the vial of "Interleukin 10 (IL10), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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