Mouse Interferon stimulated gene 15 Monoclonal Antibody | anti-ISG15 antibody
Mouse Anti Human Interferon stimulated gene 15
Applications
ELISA, Western Blot
Purity
ISG15 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Interferon stimulated gene 15; Monoclonal Antibody; Mouse Anti Human Interferon stimulated gene 15; ISG15 Antibody; Mouse Anti Human; ISG15; G1P2; IFI15; UCRP; Interferon-induced 17 kDa protein precursor; ISG15 Ubiquitin-like modifier; anti-ISG15 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 heavy chain and kappa light chain
Clone Number
P3E5AT
Purity/Purification
ISG15 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence Length
157
Applicable Applications for anti-ISG15 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ISG15 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Immunogen
Anti-human ISG15 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ISG15 amino acids 1-157 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-ISG15 antibody
Introduction: ISG15 ubiquitin-like Modifier is conjugated to the intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the ub conjugation pathway at the level of a specific E2 enzyme. ISG15 protein targets include STAT1, SERPINA 3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MXA, and RIG-1. Deconjugated by USP18/UBP43 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 serves as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 plays a role in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing ifn-gamma secretion by monocytes and macrophages. ISG15 displays a
Product Categories/Family for anti-ISG15 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,472 Da
NCBI Official Full Name
ISG15
UniProt Protein Name
ISG15
Protein Family
UniProt Entry Name
M1RFN1_9CETA
Similar Products
Product Notes
The ISG15 (Catalog #AAA140275) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interferon stimulated gene 15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ISG15 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the ISG15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon stimulated gene 15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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