Rabbit anti-Human, mouse p19 Polyclonal Antibody | anti-CDKN2D antibody

p19 Antibody (N-term)

Cat. Num. AAA9206464

• Gene Names: CDKN2D; p19; INK4D; p19-INK4D
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: p19; Polyclonal Antibody; p19 Antibody (N-term); Cyclin-dependent kinase 4 inhibitor D; p19-INK4d; CDKN2D; anti-CDKN2D antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This p19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human p19.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 15-43
• Sequence Length: 166

• Applicable Applications for anti-CDKN2D antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of p19 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). p19 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(p19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the p19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(p19 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDKN2D antibody

p19 is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis.

Product Categories/Family for anti-CDKN2D antibody

Cancer; Cell biology

References

Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Zhang, W., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(5):414-428(2009)
Agarwal, S.K., et al. J. Clin. Endocrinol. Metab. 94(5):1826-1834(2009)
Goode, E.L., et al. Cancer Epidemiol. Biomarkers Prev. 18(3):935-944(2009)
Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4502753
• NCBI GeneID: 1032
• NCBI Accession #: NP_001791.1; NP_524145.1
• NCBI GenBank Nucleotide #: NM_001800.3
• UniProt Accession #: P55273; Q13102; Q6FGE9
• Molecular Weight: 17700
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor D
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• NCBI Official Symbol: CDKN2D
• NCBI Official Synonym Symbols: p19; INK4D; p19-INK4D
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor D
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor D
• Protein Family: Cyclin-dependent kinase 4 inhibitor
• UniProt Gene Name: CDKN2D
• UniProt Entry Name: CDN2D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CDKN2D: Interacts strongly with CDK4 and CDK6 and inhibits them. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Tumor suppressor; Cell cycle regulation; Inhibitor

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: response to retinoic acid; negative regulation of caspase activity; DNA synthesis during DNA repair; negative regulation of cell proliferation; response to vitamin D; sensory perception of sound; autophagic cell death; negative regulation of phosphorylation; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; cell cycle arrest; G1/S transition of mitotic cell cycle; response to UV