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Western Blot (WB) (Western Blot: Sample: HepG2 cell lysate Primary Ab: 2ug/ml Mouse Anti-Rabbit )

Mouse anti-Rabbit Interferon Alpha Monoclonal Antibody | anti-IFNa antibody

Interferon Alpha (IFNa) Monoclonal Antibody

Reactivity
Rabbit
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Interferon Alpha; Monoclonal Antibody; Interferon Alpha (IFNa) Monoclonal Antibody; IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon; Leukocytic; Leukocyte; Interferon alpha-D; anti-IFNa antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C4
Specificity
The antibody is a mouse monoclonal antibody raised against IFNa. It has been selected for its ability to recognize IFNa in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
195
Applicable Applications for anti-IFNa antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:100-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Rabbit
Organism Species
Rabbit
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035634
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: HepG2 cell lysate Primary Ab: 2ug/ml Mouse Anti-Rabbit )

Western Blot (WB) (Western Blot: Sample: HepG2 cell lysate Primary Ab: 2ug/ml Mouse Anti-Rabbit )

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
22,319 Da
NCBI Official Full Name
interferon-alpha
NCBI Official Synonym Full Names
interferon, omega 1
NCBI Official Symbol
IFNW1
NCBI Protein Information
interferon omega-1
UniProt Protein Name
Interferon omega-1
Protein Family
UniProt Gene Name
IFNW1
UniProt Entry Name
IFNW1_HUMAN

NCBI Description

The protein encoded by this gene is an interferon and possesses antiviral activity. The encoded protein binds to the interferon alpha/beta receptor but not to the interferon gamma receptor. This intronless gene has several pseudogenes spread throughout the genome. [provided by RefSeq, Nov 2015]

Uniprot Description

IFNW1: Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space

Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding

Biological Process: adaptive immune response; B cell differentiation; B cell proliferation; cell cycle arrest; cytokine and chemokine mediated signaling pathway; defense response to virus; humoral immune response; natural killer cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to exogenous dsRNA; response to virus; T cell activation during immune response

Research Articles on IFNa

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Product Notes

The IFNa ifnw1 (Catalog #AAA2032055) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Interferon Alpha (IFNa) Monoclonal Antibody reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:100-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IFNa ifnw1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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