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Western Blot (WB) ()

Mouse Inter alpha Trypsin Inhibitor Monoclonal Antibody | anti-AMBP antibody

Inter alpha Trypsin Inhibitor antibody

Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Applications
Western Blot, Immunohistochemistry
Purity
Inter alpha Trypsin Inhibitor antibody was purified by ammonium sulfate precipitation
Synonyms
Inter alpha Trypsin Inhibitor; Monoclonal Antibody; Inter alpha Trypsin Inhibitor antibody; Monoclonal Inter alpha Trypsin Inhibitor; Anti-Inter alpha Trypsin Inhibitor; ITIH1; inter-alpha-trypsin inhibitor heavy chain 1; BOS_20950; H1P; IATIH; IGHEP1; inter-alpha (globulin) inhibitor H1; inter-alpha (globulin) inhibitor; H1 polypeptide; inter-alpha-inhibitor heavy chain 1; inter-alpha-trypsin inhibitor complex component III; inter-alpha trypsin inhibitor; heavy chain 1; inter-alpha-trypsin inhibitor heavy chain H1; inter-alpha-trypsin inhibitor heavy-chain H1; Intin1; ITIH; Itih-1; ITI HC1; ITI-HC1; ITI heavy chain H1; serum-derived hyaluronan-associated protein; SHAP; anti-AMBP antibody
Ordering
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
40B10
Purity/Purification
Inter alpha Trypsin Inhibitor antibody was purified by ammonium sulfate precipitation
Form/Format
Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Sequence Length
51
Applicable Applications for anti-AMBP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 1:500-1000
IHC-P
Biological Significance
The inter-alpha-trypsin inhibitor (ITI, IalphaI) family, a typical and classical example for protein-glycosaminoglycan-protein (PGP) complexes, occurs constitutively in plasma at relatively high concentrations and is a result of alternate combinations of three kinds of heavy chains with a common light chain, the bikunin proteoglycan. Bikunin has two proteinase inhibitor domains and belongs to the Kunitz-type protease inhibitor family; it displays an inhibitory activity against trypsin, leukocyte elastase and plasmin. The heavy chains do not have any protease inhibitory properties but have the capacity to interact in vitro and in vivo with hyaluronic acid and this binding promotes the stability of the extra-cellular matrix. The ITI protein family is suspected of playing a key role in the extra-cellular matrix biology.
Cross-Reactivity
Human
Immunogen
Inter alpha Trypsin Inhibitor antibody was raised in Mouse using a protein purified from Human plasma as the immunogen
Preparation and Storage
Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles

Western Blot (WB)

()

Western Blot (WB) ()
Related Product Information for anti-AMBP antibody
Mouse monoclonal Inter alpha Trypsin Inhibitor antibody
Product Categories/Family for anti-AMBP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
259
UniProt Accession #
Molecular Weight
38,999 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor, partial
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP
UniProt Protein Name
Protein AMBP
Protein Family
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; heme binding; calcium oxalate binding; calcium channel inhibitor activity

Biological Process: receptor-mediated endocytosis; negative regulation of JNK cascade; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage

Research Articles on AMBP

Similar Products

Product Notes

The AMBP ambp (Catalog #AAA833473) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Inter alpha Trypsin Inhibitor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 1:500-1000 IHC-P. Researchers should empirically determine the suitability of the AMBP ambp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inter alpha Trypsin Inhibitor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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