Immunohistochemistry (IHC)
(MBS9600506 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse NCK2 Polyclonal Antibody | anti-NCK2 antibody
NCK2 Antibody
Gene Names
NCK2; GRB4; NCKbeta
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NCK2; Polyclonal Antibody; NCK2 Antibody; Cytoplasmic protein NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta; anti-NCK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NCK2 antibody detects endogenous levels of NCK2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
380
Applicable Applications for anti-NCK2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NCK2
Subcellular Location
Cytoplasm. Endoplasmic Reticulum.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog, Chicken
Similarity
Pig (88%), Bovine (89%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9600506 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9600506 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NCK2 antibody
Description: Nck2 Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Interacts with DOCK1, PINCH and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Note: This description may include information from UniProtKB.
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Subunit Structure: Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.
Post-translational Modifications: Phosphorylated.
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Subunit Structure: Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.
Post-translational Modifications: Phosphorylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42 kDa
Predicted: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
cytoplasmic protein NCK2 isoform A
NCBI Official Synonym Full Names
NCK adaptor protein 2
NCBI Official Symbol
NCK2
NCBI Official Synonym Symbols
GRB4; NCKbeta
NCBI Protein Information
cytoplasmic protein NCK2
UniProt Protein Name
Cytoplasmic protein NCK2
Protein Family
UniProt Gene Name
NCK2
UniProt Synonym Gene Names
GRB4; Nck-2
NCBI Description
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Research Articles on NCK2
1. This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression.
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Product Notes
The NCK2 nck2 (Catalog #AAA9600506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCK2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCK2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NCK2 nck2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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