Flow Cytometry (FC/FACS)
(Red: isotype control mouse IgG1|Black: irrelevant mouse IgG1 control|Blue: I7661-39D|Beta-1 integrin expression on HUVEC cells. Flow cytometric detection of human b1-integrin on HUVECs with MBS633294. Controls and anti-human b1-integrin at 0.4ug/100000 cells (1 hr 4 °C).)
Mouse anti-Human Integrin, beta1 Monoclonal Antibody | anti-ITGB1 antibody
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 1:50 Stains the extracellular domain of beta-1 integrin. Fix HUVEC cells in 4% paraformaldehyde before staining.
Functional Studies: Functions as an inhibiting antibody. Functionally tested by adhesion assay (1), chemotaxis (2), patch clamp analysis (3) and neurotoxicity induced apoptosis (4).
Flow Cytometry (FC/FACS)
(Red: isotype control mouse IgG1|Black: irrelevant mouse IgG1 control|Blue: I7661-39D|Beta-1 integrin expression on HUVEC cells. Flow cytometric detection of human b1-integrin on HUVECs with MBS633294. Controls and anti-human b1-integrin at 0.4ug/100000 cells (1 hr 4 °C).)
Flow Cytometry (FC/FACS)
(Red: isotype control mouse IgG1|Black: irrelevant mouse IgG1 control|Blue: I7661-39D|Beta-1 integrin expression on HUVEC cells. Flow cytometric detection of human b1-integrin on HUVECs with MBS633294. Controls and anti-human b1-integrin at 0.4ug/100000 cells (1 hr 4 °C).)
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.; Cell surface
Chromosomal Location of Human Ortholog: 10p11.2
Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; basement membrane; neuromuscular junction; integrin complex; receptor complex; sarcolemma; external side of plasma membrane; filopodium; cleavage furrow
Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; peptide binding; protein kinase binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding
Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; axon extension; positive regulation of apoptosis; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; regulation of immune response; cell migration; in utero embryonic development; sarcomere organization; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; stress fiber formation; calcium-independent cell-matrix adhesion; cellular defense response; blood coagulation; homophilic cell adhesion; leukocyte tethering or rolling; positive regulation of cell migration
Research Articles on ITGB1
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Product Notes
The ITGB1 itgb1 (Catalog #AAA633294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin, beta1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Neutralization. Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Functional Studies. Dilution: Flow Cytometry: 1:50 Stains the extracellular domain of beta-1 integrin. Fix HUVEC cells in 4% paraformaldehyde before staining. Functional Studies: Functions as an inhibiting antibody. Functionally tested by adhesion assay (1), chemotaxis (2), patch clamp analysis (3) and neurotoxicity induced apoptosis (4). Researchers should empirically determine the suitability of the ITGB1 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin, beta1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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