Western Blot (WB)
(Figure 1. Western blot analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Integrin beta 5/ITGB5 antigen affinity purified monoclonal antibody (Catalog # MBS1754037) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Integrin beta 5/ITGB5 at approximately 100KD. The expected band size for Integrin beta 5/ITGB5 is at 100KD. )
Mouse anti-Human Integrin beta 5/ITGB5 Monoclonal Antibody | anti-ITGB5 antibody
Anti-Integrin beta 5/ITGB5 Antibody (monoclonal, 9E2)
IHC-P: 2-5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Integrin beta 5/ITGB5 antigen affinity purified monoclonal antibody (Catalog # MBS1754037) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Integrin beta 5/ITGB5 at approximately 100KD. The expected band size for Integrin beta 5/ITGB5 is at 100KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Integrin beta 5/ITGB5 antigen affinity purified monoclonal antibody (Catalog # MBS1754037) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Integrin beta 5/ITGB5 at approximately 100KD. The expected band size for Integrin beta 5/ITGB5 is at 100KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Integrin beta 5/ITGB5 using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Integrin beta 5/ITGB5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Integrin beta 5/ITGB5 Antibody (MBS1754037) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of A549 cells using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Overlay histogram showing A549 cells stained with MBS1754037 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Integrin beta 5/ITGB5 Antibody (MBS1754037, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of A549 cells using anti-Integrin beta 5/ITGB5 antibody (MBS1754037).Overlay histogram showing A549 cells stained with MBS1754037 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Integrin beta 5/ITGB5 Antibody (MBS1754037, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. McLean, J. W., Vestal, D. J., Cheresh, D. A., Bodary, S. C. cDNA sequence of the human integrin beta 5 subunit. J. Biol. Chem. 265: 17126-17131, 1990.
3. Reynolds, L. E., Wyder, L., Lively, J. C., Taverna, D., Robinson, S. D., Huang, X., Sheppard, D., Hynes, R. O., Hodivala-Dilke, K. M. Enhanced pathological angiogenesis in mice lacking beta-3 integrin or beta-3 and beta-5 integrins. Nature Med. 8: 27-34, 2002.
NCBI and Uniprot Product Information
Uniprot Description
ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q21.2
Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex
Molecular Function: integrin binding; protein binding; receptor activity
Biological Process: integrin-mediated signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; extracellular matrix organization and biogenesis; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I
Research Articles on ITGB5
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Product Notes
The ITGB5 itgb5 (Catalog #AAA1754037) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin beta 5/ITGB5 Antibody (monoclonal, 9E2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta 5/ITGB5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human| IHC-P: 2-5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human|. Researchers should empirically determine the suitability of the ITGB5 itgb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta 5/ITGB5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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