Mouse anti-Human Integrin alphaV, beta5 Monoclonal Antibody | anti-ITGB5 antibody

Integrin alphaV, beta5

Cat. Num. AAA601117

• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: Integrin alphaV; beta5; Monoclonal Antibody; Anti -Integrin alphaV; anti-ITGB5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2Q873
• Specificity: Recognizes human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS. No preservative added.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ITGB5 antibody: ELISA (EL/EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Recommended Dilutions: ; Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4°( in 20% normal human serum then washing in medium containing 1 00/0 FRS~ 5x 1 Oe5 cells per tube were subsequently incubated with primary antibody for 30 minutes on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody. Immunocytochemistry: 1 Smg/ml for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1 % paraformaldehyde containing 0.5% Triton X-l 00 for 20 minutes. Incubation for 30 minutes at 22°(, followed by detection with a fluorescein-conjugated secondary antibody. Optimal dilutions to be determined by the researcher.
• Immunogen: Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ITGB5 antibody

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

Product Categories/Family for anti-ITGB5 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 20127446
• NCBI GeneID: 3693
• NCBI Accession #: NP_002204.2
• NCBI GenBank Nucleotide #: NM_002213.3
• UniProt Accession #: P18084; B0LPF8; B2RD70
• Molecular Weight: 88,054 Da
• NCBI Official Full Name: integrin beta-5
• NCBI Official Synonym Full Names: integrin, beta 5
• NCBI Official Symbol: ITGB5
• NCBI Protein Information: integrin beta-5
• UniProt Protein Name: Integrin beta-5
• Protein Family: Integrin
• UniProt Gene Name: ITGB5
• UniProt Entry Name: ITB5_HUMAN

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; extracellular matrix organization and biogenesis; muscle contraction; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent