Rabbit anti-Human Insulin Polyclonal Antibody | anti-INS antibody

Insulin Antibody

Cat. Num. AAA840328

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Insulin; Polyclonal Antibody; Insulin Antibody; Insulin A chain; Insulin B chain; Proinsulin; anti-INS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually.
• Immunogen: E. coli expressed recombinant human Insulin
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-INS antibody

Background: Insulin is important in stimulating the uptake of glucose from blood in the skeletal muscle and adipose tissue. It is secrered by pancreatic beta cells thromugh a glucose sensing pathway. Insulin also increases cell permeability to monosaccharides, amino acids and fatty acids. Insulin is first formed as a precursor molecule, preproinsulin which is then processed into proinsulin and finally to the mature insulin hormone. A mature form of insulin has an A chain and a B chain that are connnected by two dilsufide bonds. Defects in insulin are the cause of type-II diabetes mellitus.

NCBI and Uniprot Product Information

• NCBI GI #: 4557671
• NCBI GeneID: 3630
• NCBI Accession #: NP_000198.1
• NCBI GenBank Nucleotide #: NM_000207.2
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin preproprotein
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10