Rabbit anti-Human Insulin B Chain Polyclonal Antibody | anti-INS antibody

Insulin B Chain Antibody

Cat. Num. AAA857374

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Human
• Applications: ELISA
• Purity: Immunogen affinity purified
• Synonyms: Insulin B Chain; Polyclonal Antibody; Insulin B Chain Antibody; anti-INS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the residues of human ALP.
• Purity/Purification: Immunogen affinity purified
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
• Concentration: 100ul, 0.53mg/ml (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: ELISA (EIA)
• Application Notes: ELISA: 1:500
• Preservative: 0.05% Sodium Azide.
• Cellular Localization: Secreted
• Source Note: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human insulin B chain.
• Positive Control: Recombinannt Human Insulin B Chain
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for anti-INS antibody

Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's celis to take up glucose from the blood (including liver, muscle, and fat tissue celis), storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. Aand B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.

References

1. Ezra E., Blacher R., Udenfriend S.; "Purification and partial sequencing of human placental alkaline phosphatase."; Biochem. Biophys. Res. Commun. 116:1076-1083(1983). 2. Garattini E., Hua J.-C., Pan Y.C.E., Udenfriend S.; "Human liver alkaline phosphatase, purification and partial sequencing: homology with the placental isozyme."; Arch. Biochem. Biophys. 245:331-337(1986).

NCBI and Uniprot Product Information

• NCBI GI #: 4557671
• NCBI GeneID: 3630
• NCBI Accession #: NP_000198.1
• NCBI GenBank Nucleotide #: NM_000207.2
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 12kDa
• NCBI Official Full Name: insulin preproprotein
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.