Mouse anti-Human Inhibitor of DNA Binding 2, Dominant Negative Helix-loop-helix Protein Monoclonal Antibody | anti-ID2 antibody

Inhibitor of DNA Binding 2, Dominant Negative Helix-loop-helix Protein (ID2, ID2A, ID2H, MGC26389)

Cat. Num. AAA632550

• Gene Names: ID2; GIG8; ID2A; ID2H; bHLHb26; MGC26389
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Inhibitor of DNA Binding 2; Dominant Negative Helix-loop-helix Protein; Monoclonal Antibody; Dominant Negative Helix-loop-helix Protein (ID2; ID2A; ID2H; MGC26389); Anti -Inhibitor of DNA Binding 2; anti-ID2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C195
• Specificity: Recognizes human Inhibitor of DNA Binding 2, Dominant Negative helix-loop-helix Protein.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ID2 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Western Blot and Dot Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human Inhibitor of DNA Binding 2, Dominant Negative helix-loop-helix Protein
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-ID2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21040395
• NCBI GeneID: 3398
• UniProt Accession #: Q02363; Q53H99; Q53T66
• Molecular Weight: 14,917 Da
• NCBI Official Full Name: Inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
• NCBI Official Synonym Full Names: inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID2
• NCBI Official Synonym Symbols: GIG8; ID2A; ID2H; bHLHb26; MGC26389
• NCBI Protein Information: DNA-binding protein inhibitor ID-2; helix-loop-helix protein ID2; cell growth-inhibiting gene 8; inhibitor of differentiation 2; DNA-binding protein inhibitor ID2; class B basic helix-loop-helix protein 26
• UniProt Protein Name: DNA-binding protein inhibitor ID-2
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID2
• UniProt Synonym Gene Names: BHLHB26
• UniProt Entry Name: ID2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq]

Uniprot Description

ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: nucleoplasm; microtubule cytoskeleton; centrosome; protein complex; cytoplasm; cytosol; chromatin; nucleus

Molecular Function: protein dimerization activity; protein binding

Biological Process: regulation of lipid metabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; multicellular organismal development; cell maturation; negative regulation of transcription from RNA polymerase II promoter; entrainment of circadian clock by photoperiod; locomotor rhythm; embryonic digestive tract morphogenesis; natural killer cell differentiation; negative regulation of osteoblast differentiation; circadian regulation of gene expression; oligodendrocyte development; positive regulation of macrophage differentiation; positive regulation of astrocyte differentiation; negative regulation of B cell differentiation; Peyer's patch development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; negative regulation of transcription factor activity; olfactory bulb development; neuron fate commitment; mammary gland epithelial cell proliferation; enucleate erythrocyte differentiation; regulation of circadian rhythm; negative regulation of DNA binding; negative regulation of oligodendrocyte differentiation; negative regulation of neuron differentiation; positive regulation of fat cell differentiation; negative regulation of transcription, DNA-dependent; metanephros development; positive regulation of blood pressure