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Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Mouse Anti-Human IL19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Mouse anti-Human Interleukin 19 (IL19) Monoclonal Antibody | anti-IL19 antibody

Monoclonal Antibody to Interleukin 19 (IL19)

Gene Names
IL19; MDA1; NG.1; ZMDA1; IL-10C
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Interleukin 19 (IL19); Monoclonal Antibody; Monoclonal Antibody to Interleukin 19 (IL19); MDA1; ZMDA1; IL-10C; NG.1; Melanoma Differentiation Associated Protein-Like Protein; anti-IL19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C3
Specificity
The antibody is a mouse monoclonal antibody raised against IL19. It has been selected for its ability to recognize IL19 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL19 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunogen
Recombinant Interleukin 19 (IL19) (Leu25~Ala177)
Cross Reactivity
Human, Rat, Rabbit
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Label
None
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Mouse Anti-Human IL19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Mouse Anti-Human IL19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant IL19, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant IL19, Human.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,079 Da
NCBI Official Full Name
interleukin-19 isoform 2
NCBI Official Synonym Full Names
interleukin 19
NCBI Official Symbol
IL19
NCBI Official Synonym Symbols
MDA1; NG.1; ZMDA1; IL-10C
NCBI Protein Information
interleukin-19
UniProt Protein Name
Interleukin-19
Protein Family
UniProt Gene Name
IL19
UniProt Synonym Gene Names
ZMDA1; IL-19

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

IL19: May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis. Belongs to the IL-10 family.

Protein type: Apoptosis; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity

Biological Process: apoptosis; immune response; interleukin-6 biosynthetic process; signal transduction

Research Articles on IL19

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Product Notes

The IL19 il19 (Catalog #AAA2085891) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interleukin 19 (IL19) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 19 (IL19) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the IL19 il19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 19 (IL19), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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