ELISA
(ELISA Assay using TGF beta antibodyStandard curve of human/mouse TGF beta antibody in ELISA)
Mouse TGF beta Monoclonal Antibody | anti-TGF beta antibody
TGF beta antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
TGF beta; Monoclonal Antibody; TGF beta antibody (biotin); Monoclonal TGF beta; Anti-TGF beta; Transforming Growth Factor beta 1; TGF-b1; TGFbeta1; Anti TGF beta; anti-TGF beta antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
16TFB
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-TGF beta antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 16TFB antibody reacts with Transforming Growth Factor-beta a pleiotropic cytokine, exists in five isoforms, known as TGF-beta1-5. Homologies between isoforms range from 70-80% but no homology exists to TGF-a. TGF-beta1 is ubiquitous and the most abundant form found in lymphoid organs, while other isoforms are expressed in a more restricted distribution. The biologically active state of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-beta arise by proteolytic cleavage of longer precursors the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-beta1 are identical and differ from mouse TGF-beta1 in a single amino acid. TGF-beta1 is produced in very high levels by platelets. Other cellular sources of TGF-beta1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-beta1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-beta1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-beta1 inhibits the proliferation of T cells and NK cells and downregulates the activities of activated macrophages. TGF-beta1 blocks the anti-tumor activity of IL-2 ? bearing lymphokine-activated killer cells. Recently, TGF-beta1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-beta1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-beta1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-beta-induced regulatory T cells have been termed Ti-Treg.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
ELISA
(ELISA Assay using TGF beta antibodyStandard curve of human/mouse TGF beta antibody in ELISA)
ELISA
(ELISA Assay using TGF beta antibodyStandard curve of human/mouse TGF beta antibody in ELISA)
Related Product Information for anti-TGF beta antibody
Mouse monoclonal TGF beta antibody (biotin)
Product Categories/Family for anti-TGF beta antibody
Similar Products
Product Notes
The TGF beta (Catalog #AAA832417) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TGF beta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.25 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the TGF beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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