Flow Cytometry
(Flow Cytometric analysis using CCL2 antibodyStaining of LPS stimulated normal human peripheral blood cells with CD14 antibody (FITC) and Mouse IgG1 kappa Isotype Control (left) or CCL2 antibody (right). Cells in the monocyte gate were used for analysis.)
Mouse CCL2 Monoclonal Antibody | anti-CCL2 antibody
CCL2 antibody (allophycocyanin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CCL2; Monoclonal Antibody; CCL2 antibody (allophycocyanin); Monoclonal CCL2; Anti-CCL2; CCL-2; CCL 2; CCL2 (APC); CC chemokine ligand 2; chemokine (C-C motif) ligand 2; Monocyte chemotactic protein 1; MCP1; SCYA2; MCAF; anti-CCL2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
5D3-F7
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Sequence Length
5
Applicable Applications for anti-CCL2 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1, also known as monocyte chemotactic and activating factor and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry
(Flow Cytometric analysis using CCL2 antibodyStaining of LPS stimulated normal human peripheral blood cells with CD14 antibody (FITC) and Mouse IgG1 kappa Isotype Control (left) or CCL2 antibody (right). Cells in the monocyte gate were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CCL2 antibodyStaining of LPS stimulated normal human peripheral blood cells with CD14 antibody (FITC) and Mouse IgG1 kappa Isotype Control (left) or CCL2 antibody (right). Cells in the monocyte gate were used for analysis.)
Related Product Information for anti-CCL2 antibody
Mouse monoclonal CCL2 antibody (allophycocyanin)
Product Categories/Family for anti-CCL2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CCL2 (Catalog #AAA834053) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CCL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CCL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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