Rat anti-Human IL-7 Monoclonal Antibody | anti-IL7 antibody

Rat Anti-Human IL-7-UNLB

Cat. Num. AAA670048

• Gene Names: IL7; IL-7
• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Neutralization Assay
• Synonyms: IL-7; Monoclonal Antibody; Rat Anti-Human IL-7-UNLB; Rat Anti-Human IL-7 - Purified; Unlabeled Antibody; Rat Anti-Human IL-7; anti-IL7 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: BVD10-40F6
• Specificity: Human IL-7
• Form/Format: Purified (UNLB) Antibody

Applicable Applications for anti-IL7 antibody

ELISA-Capture – Quality tested^1-3
Immunocytochemistry – Reported in literature ⁴
Neutralization – Reported in literature ^1,5

Note – May be paired with the biotinylated clone BVD10-11C10 (MB

Application Notes

ELISA - Purified (UNLB) antibody: <=5 ug/ml

Since applications vary, you should determine the optimum working dilution for the product that is apprpriate for your specific need.

Immunogen

E. coli-expressed recombinant human IL-7

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffered salts added.
Store at 2-8°C.

Testing Data

(Standard curve generated with Rat Anti-Human IL-7-UNLB (MBS670048; Clone BVD10-40F6) and Rat Anti-Human IL-7-BIOT (MBS670051; Clone BVD10-11C10) followed by Mouse Anti-BIOT-HRP (MBS675023))

References

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

NCBI and Uniprot Product Information

• NCBI GI #: 4504677
• NCBI GeneID: 3574
• NCBI Accession #: NP_000871.1
• NCBI GenBank Nucleotide #: NM_000880.3
• UniProt Accession #: P13232; A0N0L3; Q5FBX5; Q5FBY3; Q5FBY5; Q5FBY6; Q5FBY8; Q5FBY9
• NCBI Official Full Name: interleukin-7 isoform 1
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7; OTTHUMP00000227044; OTTHUMP00000227046
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Entry Name: IL7_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.

Uniprot Description

Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-7/IL-9 family.