Mouse anti-Human IL-12 Monoclonal Antibody | anti-IL-12 antibody

Mouse Anti-Human IL-12

Cat. Num. AAA690520

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Human
• Purity: Protein A chromatography
• Synonyms: IL-12; Monoclonal Antibody; Mouse Anti-Human IL-12; Monoclonal antibody anti-human IL-12; anti-IL-12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Protein A chromatography
• Form/Format: Lyophilized
• Sequence Length: 253

• Application Notes: In a sandwich ELISA (assuming 100ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 0.2 ng/well of recombinant human IL-12 when used with PeproTech's biotinylated antigen affinity purified anti-human IL-12 as the detection antibody.ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 0.2 ng/well of recombinant human IL-12 when used with PeproTech's biotinylated antigen affinity purified anti-human IL-12 as the detection antibody.
• Antibody Generation: Monoclonal antibodies were produced in BALB/c X ICR F1 mice using recombinant hIL-12 as the immunizing antigen. IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
• Buffer: none

Related Product Information for anti-IL-12 antibody

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873.2
• NCBI GenBank Nucleotide #: NM_000882.3
• UniProt Accession #: P29459; Q96QZ1
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin-12 subunit alpha
• NCBI Official Synonym Full Names: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Ref.8

Subcellular location: Secreted.

Induction: Down-regulated in response to enterovirus 71 (EV71) infection. Ref.7

Sequence similarities: Belongs to the IL-6 superfamily.

Sequence caution: The sequence AAA59937.1 differs from that shown. Reason: Erroneous initiation.