SDS-Page
(SDS-PAGE Analysis Purified IL-10R1 Rat Monoclonal Antibody (1B1.3a). Confirmation of Integrity and Purity of Antibody.)
Rat IL-10R1 (Interleukin-10 Receptor 1) Monoclonal Antibody | anti-IL-10R1 antibody
IL-10R1, Mouse (Interleukin-10 Receptor 1) (CD210)
Gene Names
Il10ra; Il10r; CDw210; CDw210a; IL-10R1; IL-10RA; mIL-10R; AW553859
Reactivity
MouseDoes not react with Human.
Applications
Neutralization Assay, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
IL-10R1 (Interleukin-10 Receptor 1); Monoclonal Antibody; IL-10R1; Mouse (Interleukin-10 Receptor 1) (CD210); CD210 antigen; CDw210a; IBD28; IL10R alpha; IL-10R subunit 1; IL10RA IL10RIL-10R subunit alpha; interleukin 10 receptor; alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; anti-IL-10R1 antibody
Host
Rat
Reactivity
Mouse
Does not react with Human.
Does not react with Human.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
1B1.3a
Specificity
This monoclonal antibody recognizes Mouse Interleukin-10 receptor 1 (IL-10R1), a cell surface glycoprotein of approximately 110KDa. Its epitope is localized in the ligand-binding domain in the extracellular region of Mouse IL-10R1. This antibody blocks the binding of IL-10 to its receptor. IL-10R1 is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Binding of IL-10 to its receptor initiates JAK/STAT signaling that leads to B cell proliferation and inhibition of macrophage activation.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
575
Applicable Applications for anti-IL-10R1 antibody
Neutralization Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Neutralization Studies: Order Ab without Azide
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 0.5-1ug/ml
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 0.5-1ug/ml
Positive Control
MPBMC's. Spleen, Thymus, Lymph Node, Placenta, Lung or Liver.
Immunogen
Purified recombinant extracellular region of Mouse IL10RA
Cellular Localization
Cell Surface
Hu-chromosome Location
9 A5.2; 9 24.84 cM (Mouse)
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified IL-10R1 Rat Monoclonal Antibody (1B1.3a). Confirmation of Integrity and Purity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified IL-10R1 Rat Monoclonal Antibody (1B1.3a). Confirmation of Integrity and Purity of Antibody.)
References
1. Vieira PL, et al. 2004. J. Immunol. 172:5986. (Neutralization). 2. Wang W, et al. 2004. FASEB J. 18:1043. (Neutralization)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90-110kDa
NCBI Official Full Name
interleukin-10 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 10 receptor, alpha
NCBI Official Symbol
Il10ra
NCBI Official Synonym Symbols
Il10r; CDw210; CDw210a; IL-10R1; IL-10RA; mIL-10R; AW553859
NCBI Protein Information
interleukin-10 receptor subunit alpha
UniProt Protein Name
Interleukin-10 receptor subunit alpha
UniProt Gene Name
Il10ra
UniProt Synonym Gene Names
Il10r; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
UniProt Entry Name
I10R1_MOUSE
Uniprot Description
IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: interleukin-10 binding; interleukin-10 receptor activity
Biological Process: cytokine and chemokine mediated signaling pathway
Research Articles on IL-10R1
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Product Notes
The IL-10R1 il10ra (Catalog #AAA4381528) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The IL-10R1, Mouse (Interleukin-10 Receptor 1) (CD210) reacts with Mouse Does not react with Human. and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10R1 (Interleukin-10 Receptor 1) can be used in a range of immunoassay formats including, but not limited to, Neutralization Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Neutralization Studies: Order Ab without Azide FC/FACS: 0.5-1ug/million cells in 0.1ml IF: 0.5-1ug/ml. Researchers should empirically determine the suitability of the IL-10R1 il10ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10R1 (Interleukin-10 Receptor 1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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