SCO2 recombinant protein

SCO2, 42-266aa, Human, His tag, E Coli

Cat. Num. AAA203996

• Gene Names: SCO2; MYP6; SCO1L; CEMCOX1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SCO2; 42-266aa; Human; His tag; E Coli; Protein SCO2 homolog mitochondrial; SCO1L; SCO2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGPAETGGQG QPQGPGLRTR LLITGLFGAG LGGAWLALRA EKERLQQQKR TEALRQAAVG QGDFHLLDHR GRARCKADFR GQWVLMYFGF THCPDICPDE LEKLVQVVRQ LEAEPGLPPV QPVFITVDPE RDDVEAMARY VQDFHPRLLG LTGSTKQVAQ ASHSYRVYYN AGPKDEDQDY IVDHSIAIYL LNPDGLFTDY YGRSRSAEQI SDSVRRHMAA FRSVLS
• Sequence Length: 266

• Applicable Applications for SCO2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SCO2 recombinant protein

SCO2 protein belongs to the SCO1/2 family of proteins. Both SCO1 and SCO2 proteins are located on the inner membrane of the mitochondria and plays a crucial role in copper insertion or transport to the active site of cytochrome c oxidase (COX). Defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC). This disease is characterized by hypertrophic cardiomyopathy, lactic acidosis, and gliosis. Heart and skeletal muscle show reductions in cytochrome c oxidase (COX) activity, whereas liver and fibroblasts show mild COX deficiencies. Recombinant human SCO2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SCO2 recombinant protein

Metabolism

References

Meister G., et al. (2001) EMBO J. 20:2304-2314; Talbot K., et al. (1998) Hum. Mol. Genet. 7:2149-2156;

NCBI and Uniprot Product Information

• NCBI GI #: 153791313
• NCBI GeneID: 9997
• NCBI Accession #: NP_005129
• NCBI GenBank Nucleotide #: NM_005138.2
• UniProt Accession #: O43819; Q3T1B5; Q9UK87
• Molecular Weight: 27.4kDa (246aa), confirmed by MALDI-TOF
• NCBI Official Full Name: protein SCO2 homolog, mitochondrial
• NCBI Official Synonym Full Names: SCO2, cytochrome c oxidase assembly protein
• NCBI Official Symbol: SCO2
• NCBI Official Synonym Symbols: MYP6; SCO1L; CEMCOX1
• NCBI Protein Information: protein SCO2 homolog, mitochondrial
• UniProt Protein Name: Protein SCO2 homolog, mitochondrial
• Protein Family: SCO2-like protein
• UniProt Gene Name: SCO2

NCBI Description

Cytochrome c oxidase (COX) catalyzes the transfer of electrons from cytochrome c to molecular oxygen, which helps to maintain the proton gradient across the inner mitochondrial membrane that is necessary for aerobic ATP production. Human COX is a multimeric protein complex that requires several assembly factors; this gene encodes one of the COX assembly factors. The encoded protein is a metallochaperone that is involved in the biogenesis of cytochrome c oxidase subunit II. Mutations in this gene are associated with fatal infantile encephalocardiomyopathy and myopia 6. [provided by RefSeq, Oct 2014]

Uniprot Description

Acts as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2).