Typical Testing Data/Standard Curve (for reference only)
Rat Beta-Endorphin (bEP) ELISA Kit | bEP elisa kit
Rat Beta-Endorphin (bEP) ELISA Kit
Reactivity
Rat
Synonyms
Beta-Endorphin (bEP); Rat Beta-Endorphin (bEP) ELISA Kit; b-EP; bEP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP).
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
Sequence Length
31
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cerebrospinal Fluid, Cell Culture Supernates and Other Biological Fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
< 4.83pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for bEP elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
NCBI and Uniprot Product Information
Uniprot Description
Beta-endorphin and Met-enkephalin are endogenous opiates.
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Product Notes
The Rat bEP pomc (Catalog #AAA2700307) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2700307 ELISA Kit recognizes Rat bEP. It is sometimes possible for the material contained within the vial of "Beta-Endorphin (bEP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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