Mouse anti-Human, Mouse IkB-alpha Monoclonal Antibody | anti-NFKBIA antibody

IkB-alpha (N-terminus) Antibody

Cat. Num. AAA9215378

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: IkB-alpha; Monoclonal Antibody; IkB-alpha (N-terminus) Antibody; Purified Mouse Monoclonal Antibody (Mab); I kappa B alpha; I(Kappa)B(alpha); I-kappa-B-alpha; IkappaBalpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha.; anti-NFKBIA antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Sequence Length: 317

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:1000
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of IkB-alpha(N-terminus) in Jurkat and Hela cell lysates using IkB-alpha(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)

Related Product Information for anti-NFKBIA antibody

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Product Categories/Family for anti-NFKBIA antibody

Cancer; Cell Biology; Immunology; Signal Transduction

References

Haskill S.,et al.Cell 65:1281-1289(1991). Jungnickel B.,et al.J. Exp. Med. 191:395-402(2000). Liu B.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases. Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35609
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.