Goat Syndecan 4 ELISA Kit | SDC4 elisa kit

Goat Syndecan 4 ELISA Kit

Cat. Num. AAA736551

• Gene Names: SDC4; SYND4
• Reactivity: Goat
• Synonyms: Syndecan 4; Goat Syndecan 4 ELISA Kit; SDC4 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This SDC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat SDC4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDC4 elisa kit

Principle of the Assay: SDC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDC4 antibody and an SDC4-HRP conjugate. The assay sample and buffer are incubated together with SDC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDC4 concentration since SDC4 from samples and SDC4-HRP conjugate compete for the anti-SDC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by SDC4 from the sample, fewer sites are left to bind SDC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SDC4 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 38201675
• NCBI GeneID: 6385
• NCBI Accession #: NP_002990.2
• NCBI GenBank Nucleotide #: NM_002999.3
• UniProt Accession #: P31431; O00773; Q16833; Q53FN9; Q6FGN3
• Molecular Weight: 21,642 Da
• NCBI Official Full Name: syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: SDC4
• NCBI Official Synonym Symbols: SYND4
• NCBI Protein Information: syndecan-4; amphiglycan; ryudocan amphiglycan; ryudocan core protein; syndecan proteoglycan 4; syndecan 4 (amphiglycan, ryudocan)
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: SDC4
• UniProt Synonym Gene Names: SYND4
• UniProt Entry Name: SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: lysosomal lumen; costamere; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; protein kinase C binding; fibronectin binding

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; pathogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of protein kinase activity; carbohydrate metabolic process; positive regulation of stress fiber formation; retinoid metabolic process