Rabbit anti-Human MEF2C Polyclonal Antibody | anti-MEF2C antibody

MEF2C, Phosphorylated (Thr20) (Myocyte-specific Enhancer Factor 2C) (Azide free) (HRP)

Cat. Num. AAA6489005

• Gene Names: MEF2C; DEL5q14.3; C5DELq14.3
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MEF2C; Polyclonal Antibody; Phosphorylated (Thr20) (Myocyte-specific Enhancer Factor 2C) (Azide free) (HRP); anti-MEF2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MEF2C when phosphorylated at Thr20.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MEF2C antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr20 of human MEF2C (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-MEF2C-T20 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Product Categories/Family for anti-MEF2C antibody

Antibodies; Transcription Factors

References

1. Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). 2. Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). 3. Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). 4. Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). 5. Krainc, D., et al., Genomics 29(3):809-811 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 19923215
• NCBI GeneID: 4208
• NCBI Accession #: NP_002388
• NCBI GenBank Nucleotide #: NM_002397
• UniProt Accession #: Q06413; C9JMZ0; D7F7N5; F8W7V7
• Molecular Weight: 50,242 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2C isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2C
• NCBI Official Symbol: MEF2C
• NCBI Official Synonym Symbols: DEL5q14.3; C5DELq14.3
• NCBI Protein Information: myocyte-specific enhancer factor 2C; MADS box transcription enhancer factor 2, polypeptide C
• UniProt Protein Name: Myocyte-specific enhancer factor 2C
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2C
• UniProt Entry Name: MEF2C_HUMAN

Uniprot Description

MEF2C: transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: nucleoplasm; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; miRNA binding; transcription activator binding; DNA binding; AT DNA binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; muscle development; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; regulation of neurotransmitter secretion; stress-activated MAPK cascade; regulation of synaptic plasticity; toll-like receptor 3 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; positive regulation of MAP kinase activity; B cell receptor signaling pathway; regulation of neuron apoptosis; negative regulation of ossification; positive regulation of B cell proliferation; chondrocyte differentiation; negative regulation of neuron apoptosis; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; endochondral ossification; nervous system development; skeletal muscle development; monocyte differentiation; response to virus; myotube differentiation; positive regulation of skeletal muscle development; muscle cell fate determination; toll-like receptor 2 signaling pathway; muscle cell differentiation; regulation of synaptogenesis; neuron development; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; toll-like receptor 9 signaling pathway; B cell proliferation; transcription from RNA polymerase II promoter; regulation of synaptic transmission, glutamatergic; apoptosis; neuron migration; ventricular cardiac muscle cell differentiation; regulation of synaptic activity; negative regulation of transcription from RNA polymerase II promoter; palate development; toll-like receptor 10 signaling pathway; learning and/or memory; B cell homeostasis; regulation of transcription, DNA-dependent; melanocyte differentiation; regulation of megakaryocyte differentiation; germinal center formation; neural crest cell differentiation; heart looping; negative regulation of epithelial cell proliferation; blood vessel development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; embryonic viscerocranium morphogenesis; positive regulation of bone mineralization; humoral immune response; regulation of germinal center formation; positive regulation of myoblast differentiation; osteoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of osteoblast differentiation; neuron morphogenesis during differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; blood vessel remodeling; platelet formation; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation

Disease: Mental Retardation, Autosomal Dominant 20