Testing Data
Mouse anti-Human IgG Monoclonal Antibody | anti-IgG antibody
anti-human IgG-Biotin
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
IgG; Monoclonal Antibody; anti-human IgG-Biotin; Monoclonal anti-human IgG/Biotin; anti-IgG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ICO-97
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
476
Applicable Applications for anti-IgG antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Performance
Five x 105 human IgG1k-coated microparticles (Inquire) were incubated 45 minutes on ice with 80 ul of anti-human IgG/Biotin at 10 ug/ml. Tubes were washed twice and incubated with 2 degree reagent Streptavidin R-Phycoerythrin after which they were washed three times, fixed and analyzed by FACS. Microparticles stained positive with a mean shift of 2.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human IgG
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-IgG antibody
Information: Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. Antibody ICO-97 recognizes human IgG in EIA and cell surface human IgG on B cells in FACS.
References
Basic and Clinical Immunology, Seventh edition (D. P. Sites & A. I. Terr, eds.) Appleton & Lange., Norwalk, CT (1991).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The IgG (Catalog #AAA666120) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human IgG-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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