Mouse anti-Horse IgE Monoclonal Antibody | anti-IgE antibody

MOUSE ANTI HORSE IgE

Cat. Num. AAA226022

• Reactivity: Horse
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot, ELISA
• Synonyms: IgE; Monoclonal Antibody; MOUSE ANTI HORSE IgE; IgE antibody|7H2; anti-IgE antibody

• Host: Mouse
• Reactivity: Horse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H2
• Specificity: IgE
• Form/Format: Purified IgG, liquid. Phosphate buffered saline; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-IgE antibody: Immunohistochemistry (IHC)-Frozen, Immunohistochemistry (IHC)-Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1/40-1/4002; ELISA: This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with as the detection reagent.; Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.; Western Blotting: Clone 7H2 has been demonstrated to detect a band of approximately 200 kDa in non-reducing conditions. This clone is not suitable for use in western blotting under reducing conditions. For western blotting applications it is recommended to use clone 3H10
• Immunogen: Equine IgE
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3)
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line
• Carrier Free: Yes
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.Storage in frost-free freezers is not recommended.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

ELISA (EIA)

(Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE antibody, clone 7H2 followed by Rabbit F(ab')2 anti Mouse IgG:HRP .)

Related Product Information for anti-IgE antibody

Mouse anti Horse IgE antibody, clone 7H2 recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 7H2 demonstrates a single major band of approximately 200 kDa under non-reducing conditions that corresponds with the expected molecular weight of the complete equine IgE molecule (Wilson, D.A. et al. 2006).
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following worm infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A., et al. 2012).

References

1. Wilson, D. A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.Vet Immunol Immunopathol. 112: 156-70.