Typical Testing Data/Standard Curve (for reference only)
Rabbit LAT ELISA Kit | LAT elisa kit
Rabbit LAT (Linker For Activation of T-cell) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LAT. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LAT. You can calculate the concentration of LAT in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
LAT: an adaptor protein involved in T-cell antigen receptor (TCR) signal transduction pathway. Plays an important role downstream of protein tyrosine kinases. Essential for TCR-mediated Ras-Erk activation and calcium flux.
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: signalosome; mast cell granule; integral to membrane; plasma membrane; immunological synapse; intercellular junction; lipid raft
Molecular Function: protein binding; SH3/SH2 adaptor activity; protein kinase binding
Biological Process: integrin-mediated signaling pathway; platelet activation; T cell activation; positive regulation of signal transduction; calcium-mediated signaling; mast cell degranulation; T cell receptor signaling pathway; Ras protein signal transduction; innate immune response; immune response; gene expression; blood coagulation; inflammatory response; lymphocyte homeostasis; regulation of T cell activation
Research Articles on LAT
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Product Notes
The Rabbit LAT lat (Catalog #AAA2509030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509030 ELISA Kit recognizes Rabbit LAT. It is sometimes possible for the material contained within the vial of "LAT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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