ELISA (EIA)
(Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Mouse anti-Horse IgE Monoclonal Antibody | anti-IgE antibody
MOUSE ANTI HORSE IgE
Reactivity
Horse
Applications
Immunohistochemistry, Western Blot, ELISA
Synonyms
IgE; Monoclonal Antibody; MOUSE ANTI HORSE IgE; IgE antibody|3H10; anti-IgE antibody
Host
Mouse
Reactivity
Horse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H10
Specificity
IgE
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-IgE antibody
Immunohistochemistry (IHC)-Frozen, Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1/1000-1/10,000
ELISA: This product is suitable for use in indirect ELISA applications.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting: Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006).
ELISA: This product is suitable for use in indirect ELISA applications.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting: Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006).
Immunogen
Equine IgE
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3X myeloma cell line
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
ELISA (EIA)
(Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
ELISA (EIA)
(Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Related Product Information for anti-IgE antibody
Mouse anti Horse IgE, clone 3H10, recognizes native equine IgE and does not cross react with equine IgM, IgA or IgG.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations.
IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following infection.
Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A. et al. 2012).
References
1. Wilson, D.A. et al. (2006) Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.Vet Immunol Immunopathol.112: 56-70.
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Product Notes
The IgE (Catalog #AAA226020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HORSE IgE reacts with Horse and may cross-react with other species as described in the data sheet. AAA Biotech's IgE can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC)-Frozen, Western Blot (WB), ELISA (EIA). ELISA: 1/1000-1/10,000 ELISA: This product is suitable for use in indirect ELISA applications. Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Western Blotting: Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 3H10 demonstrates a band of approximately 80 kDa under reducing conditions and a band of approximately 200 kDa under non-reducing conditions which correspond with the expected molecular weight of equine Ig epsilon chain and the complete equine IgE molecule, respectively (Wilson, D.A. et al. 2006). Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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