Mouse anti-Human IgA (Immunoglobulin Alpha Heavy Chain) Monoclonal Antibody | anti-IGHA1 antibody

IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439405

• Gene Names: IGHA1; IgA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: IgA (Immunoglobulin Alpha Heavy Chain); Monoclonal Antibody; IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody; A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IGHA1; IGHA2; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 1; Immunoglobulin Heavy Constant Alpha 2; anti-IGHA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM187
• Specificity: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 353

• Applicable Applications for anti-IGHA1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (1-2ug/million cells in 0.1ml); Immunofluorescence (0.5-1.0ug/ml 30 min RT); Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasm, Cell Surface and Secreted
• Immunogen: Purified human IgA
• Hu-Chromosome Location: 14q32.33
• Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Biewenga J. et al. Monoclonal antibodies against different domains of human IgA: Specificities determined by immunoblotting and haemagglutinationinhibition. Mol. Immunol. 23: 761 767 (1986). Biewenga J. et al. Domain specificity and assay specificity of monoclonal antibodies against human IgA. Adv. Exp. Med. Biol. 216B: 1239 1249 (1987). Mestecky J. et al. Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study. J. Immunol. Meth. 193: 103 148 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 113584
• NCBI GeneID: 3493
• UniProt Accession #: P01876; P01877
• Molecular Weight: 50-75kDa
• NCBI Official Full Name: Ig alpha-1 chain C region
• NCBI Official Synonym Full Names: immunoglobulin heavy constant alpha 1
• NCBI Official Symbol: IGHA1
• NCBI Official Synonym Symbols: IgA1
• UniProt Protein Name: Ig alpha-1 chain C region
• Protein Family: Ig alpha-1 chain C region
• UniProt Gene Name: IGHA1
• UniProt Entry Name: IGHA1_HUMAN

Uniprot Description

IGHA1: Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. A chromosomal aberration involving IGHA1 is found in multiple myeloma (MM) cell lines. Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein.

Protein type: Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: external side of plasma membrane; extracellular region; extracellular space

Molecular Function: antigen binding

Biological Process: antibacterial humoral response; B cell receptor signaling pathway; complement activation, classical pathway; glomerular filtration; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of B cell activation; receptor-mediated endocytosis; retinal homeostasis