Typical Testing Data/Standard Curve (for reference only)
Human dermato-pontin, DPT ELISA Kit | DPT elisa kit
Human dermato-pontin, DPT ELISA Kit
Gene Names
DPT; TRAMP
Reactivity
Human
Synonyms
dermato-pontin; DPT; Human dermato-pontin; DPT ELISA Kit; DPT elisa kit
Reactivity
Human
Sequence Length
201
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ug/ml-7ug/ml
Sensitivity
0.02ug/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DPT elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Dermatan Sulfate (also known as DS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human DS antibody. DS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human DS Antibody is added and binds to DS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human DS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human DS antibody. DS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human DS Antibody is added and binds to DS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human DS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,005 Da
NCBI Official Full Name
dermatopontin
NCBI Official Synonym Full Names
dermatopontin
NCBI Official Symbol
DPT
NCBI Official Synonym Symbols
TRAMP
NCBI Protein Information
dermatopontin
UniProt Protein Name
Dermatopontin
Protein Family
UniProt Gene Name
DPT
UniProt Synonym Gene Names
TRAMP
NCBI Description
Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008]
Uniprot Description
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation ().
Research Articles on DPT
Similar Products
Product Notes
The Human DPT dpt (Catalog #AAA164404) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA164404 ELISA Kit recognizes Human DPT. It is sometimes possible for the material contained within the vial of "dermato-pontin, DPT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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