Immunofluorescence (IF)
(Immunofluorescence staining of Mouse ICAM1 in Raw264.7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse ICAM1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.)
Rabbit anti-Mouse ICAM-1 Monoclonal Antibody | anti-ICAM1 antibody
Recombinant Anti-ICAM-1 Antibody, Rabbit Monoclonal
ELISA (Det): 1:1000-1:10,000
ELISA (Det): This antibody will detect Mouse ICAM-1/CD54 in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
Immunofluorescence (IF)
(Immunofluorescence staining of Mouse ICAM1 in Raw264.7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse ICAM1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Mouse ICAM1 in Raw264.7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse ICAM1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.)
NCBI and Uniprot Product Information
Uniprot Description
ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.
Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily
Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; immunological synapse; external side of plasma membrane; lipid raft
Molecular Function: integrin binding; protein complex binding
Biological Process: regulation of cell adhesion; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; sensory perception of sound; positive regulation of actin filament polymerization; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; cell adhesion; acute inflammatory response to antigenic stimulus
Research Articles on ICAM1
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Product Notes
The ICAM1 icam1 (Catalog #AAA8111571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-ICAM-1 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ICAM-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) (Det), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 ELISA (Det): 1:1000-1:10,000 ELISA (Det): This antibody will detect Mouse ICAM-1/CD54 in ELISA pair set. In a sandwich ELISA, it can be used as detection antibody when paired with. Researchers should empirically determine the suitability of the ICAM1 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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