Rabbit anti-Rat ICAM-1 Monoclonal Antibody | anti-ICAM1 antibody

Recombinant Anti-ICAM-1 Antibody (PE), Rabbit Monoclonal

Cat. Num. AAA8112629

• Gene Names: Icam1; CD54; ICAM
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: ICAM-1; Monoclonal Antibody; Recombinant Anti-ICAM-1 Antibody (PE); Rabbit Monoclonal; ICAM-1/CD54 Antibody (PE); Rabbit MAb; intercellular adhesion molecule 1; anti-ICAM1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 037
• Specificity: Rat ICAM-1/CD54
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 2ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-ICAM1 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat ICAM-1/CD54 protein
• Conjugation: PE
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat ICAM-1/CD54 (rR ICAM-1/CD54) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Analysis of Rat ICAM1(CD54) expression on spleen lymphocytes. Crossreactivity on both rat and mouse splenocytes is observed. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.)

Related Product Information for anti-ICAM1 antibody

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Product Categories/Family for anti-ICAM1 antibody

Neuroinflammation

References

Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.

NCBI and Uniprot Product Information

• NCBI GI #: 6981068
• NCBI GeneID: 25464
• NCBI Accession #: NP_037099.1
• NCBI GenBank Nucleotide #: NM_012967.1
• UniProt Accession #: Q00238
• Molecular Weight: 60,142 Da
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: Icam1
• NCBI Official Synonym Symbols: CD54; ICAM
• NCBI Protein Information: intercellular adhesion molecule 1; ICAM-1
• UniProt Protein Name: Intercellular adhesion molecule 1
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: Icam1
• UniProt Synonym Gene Names: Icam-1; ICAM-1
• UniProt Entry Name: ICAM1_RAT

NCBI Description

cell adhesion molecule; ligand for leukocyte adhesion molecule LFA-1 [RGD, Feb 2006]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein complex binding

Biological Process: positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to lipopolysaccharide; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cell-cell adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; acute inflammatory response to antigenic stimulus; regulation of cell adhesion; response to drug; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; response to amphetamine; cell aging; response to amino acid stimulus; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; response to hypoxia; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; response to ionizing radiation; leukocyte migration; positive regulation of GTPase activity