Rabbit anti-Rat ICAM-1 Polyclonal Antibody | anti-ICAM1 antibody

Anti-ICAM-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112634

• Gene Names: Icam1; CD54; ICAM
• Reactivity: Rat
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ICAM-1; Polyclonal Antibody; Anti-ICAM-1 Antibody; Rabbit Polyclonal; ICAM-1/CD54 Antibody; Rabbit PAb; Antigen Affinity Purified; intercellular adhesion molecule 1; anti-ICAM1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat ICAM-1/CD54
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ICAM1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:10,000; IP: 5-10uL/mg of lysate
• Immunogen: Recombinant Rat ICAM-1/CD54 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat ICAM-1/CD54 (rR ICAM-1/CD54). ICAM-1/CD54 specific IgG was purified by affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ICAM1 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa Observed band size:65 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of rat ICAM1 in rat kidney with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of rat ICAM1 in rat liver with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(ratICAM1 was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate 2 uL anti-ratICAM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratICAM1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa Observed band size :55 kDa)

Related Product Information for anti-ICAM1 antibody

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Product Categories/Family for anti-ICAM1 antibody

Neuroinflammation

References

Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.

NCBI and Uniprot Product Information

• NCBI GI #: 6981068
• NCBI GeneID: 25464
• NCBI Accession #: NP_037099.1
• NCBI GenBank Nucleotide #: NM_012967.1
• UniProt Accession #: Q00238
• Molecular Weight: 60,142 Da
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: Icam1
• NCBI Official Synonym Symbols: CD54; ICAM
• NCBI Protein Information: intercellular adhesion molecule 1; ICAM-1
• UniProt Protein Name: Intercellular adhesion molecule 1
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: Icam1
• UniProt Synonym Gene Names: Icam-1; ICAM-1
• UniProt Entry Name: ICAM1_RAT

NCBI Description

cell adhesion molecule; ligand for leukocyte adhesion molecule LFA-1 [RGD, Feb 2006]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein complex binding

Biological Process: positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to lipopolysaccharide; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cell-cell adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; acute inflammatory response to antigenic stimulus; regulation of cell adhesion; response to drug; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; response to amphetamine; cell aging; response to amino acid stimulus; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; response to hypoxia; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; response to ionizing radiation; leukocyte migration; positive regulation of GTPase activity