Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSPBP1 protein (Right) or empty vector (Left) detected with HSPBP1 antibody)
Mouse HSPBP1 Monoclonal Antibody | anti-HSPBP1 antibody
HSPBP1 antibody
Gene Names
HSPBP1; FES1
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
HSPBP1 antibody was purified by affinity chromatography.
Synonyms
HSPBP1; Monoclonal Antibody; HSPBP1 antibody; Monoclonal HSPBP1; Anti-HSPBP1; HSPA binding protein cytoplasmic cochaperone 1; Heat shock 70kDa; FES1; HspBP1; Hsp70 binding protein 2; Hsp70 interacting protein 1; HSPBP 1; HSPBP-1; anti-HSPBP1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2D1
Purity/Purification
HSPBP1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
359
Applicable Applications for anti-HSPBP1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
IF: 1:100
WB: 1:2000
Biological Significance
HSPBP1 inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. HSPBP1 interferes with ubiquitin ation mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
Cross-Reactivity
Human
Immunogen
HSPBP1 antibody was raised in mouse using a full length recombinant protein of human HSPBP1 (NP_036399) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSPBP1 protein (Right) or empty vector (Left) detected with HSPBP1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSPBP1 protein (Right) or empty vector (Left) detected with HSPBP1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HSPBP1 protein using HSPBP1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant HSPBP1 protein using HSPBP1 antibody)
Related Product Information for anti-HSPBP1 antibody
Mouse monoclonal HSPBP1 antibody
Product Categories/Family for anti-HSPBP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,516 Da
NCBI Official Full Name
hsp70-binding protein 1 isoform 2
NCBI Official Synonym Full Names
HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
NCBI Official Symbol
HSPBP1
NCBI Official Synonym Symbols
FES1
NCBI Protein Information
hsp70-binding protein 1
UniProt Protein Name
Hsp70-binding protein 1
Protein Family
UniProt Gene Name
HSPBP1
UniProt Synonym Gene Names
HSPBP; HspBP1; HspBP2
UniProt Entry Name
HPBP1_HUMAN
Uniprot Description
HSPBP1: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.42
Molecular Function: enzyme inhibitor activity; protein binding
Biological Process: protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of catalytic activity
Research Articles on HSPBP1
Similar Products
Product Notes
The HSPBP1 hspbp1 (Catalog #AAA533892) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSPBP1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the HSPBP1 hspbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPBP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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