Matrix Metalloproteinase 16 (MMP16) Recombinant Protein | MMP16 recombinant protein

Recombinant Matrix Metalloproteinase 16 (MMP16)

Cat. Num. AAA2018530

• Gene Names: MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Matrix Metalloproteinase 16 (MMP16); Recombinant Matrix Metalloproteinase 16 (MMP16); MMP16 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR QAC- AILRREMFVF KDQWFWRVRN NRVMDGYPMQ ITYFWRGLPP SIDAVYENSD GNFVFFKGNK YWVFKDTTLQ PGYPHDLITL GSGIPPHGID SAIWWEDVGK TYFFKGDRYW RYSEEMKTMD PGYPKPITVW KGIPESPQGA FVHKENGFTY FYKGKEYWKF NNQILKVEPG YPRSILKDFMGCDGPTDRVK EGHSPPDDVD IVIKLDN
• Sequence Length: 607

• Applicable Applications for MMP16 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala351~Asn557 (Accession # P51512) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein; Extracellular side. Secreted, extracellular space, extracellular matrix. Cell surface
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13027802
• NCBI GeneID: 4325
• NCBI Accession #: NP_005932.2
• NCBI GenBank Nucleotide #: NM_005941.4
• UniProt Accession #: P51512; Q14824; Q52H48; B2RAN7
• Molecular Weight: 29.1kDa
• NCBI Official Full Name: matrix metalloproteinase-16
• NCBI Official Synonym Full Names: matrix metallopeptidase 16 (membrane-inserted)
• NCBI Official Symbol: MMP16
• NCBI Official Synonym Symbols: MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• NCBI Protein Information: matrix metalloproteinase-16; MMP-16; MT3MMP; MTMMP3; MT-MMP 3; Putative transmembrane protein C8orf57; membrane-type matrix metalloproteinase 3; membrane-type-3 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-16
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP16
• UniProt Synonym Gene Names: MMPX2; MMP-16; MT-MMP 3; MTMMP3; MT3-MMP; MT3MMP
• UniProt Entry Name: MMP16_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]

Uniprot Description

MMP16: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: proteinaceous extracellular matrix; cell surface; integral to plasma membrane; Golgi lumen; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; embryonic cranial skeleton morphogenesis; proteolysis; endochondral ossification